HotStar DNA Polymerase

bulk pack for IVD

PC11, PC12, Hotstar DNA Polymerase, E star Taq DNA Polymerase, Anti body modifed Hotstar DNA Polymerase, suitable for IVD use

Product Description

The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality.

Taq DNA polymerase: Has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′ – 5′ exonuclease activity. The extending speed is 1 kb/30 sec. There is an “A” on 3′ end. The PCR product can be cloned in TA vector. 

Concentration: 5 u/μl

Package: Bulk

Storage: Store at -20°C

Quality Control

  • Functional absence of double and single-stranded endonuclease activity;
  • Purity>99% test by SDS gel electrophoresis;
  • Each lot of HotStar Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA;
  • Retain full activity at room temperature for one week;
  • No host DNA residue.
HotStar DNA Polymerase bulk pack for IVD

HotStar DNA Polymerase bulk pack for IVD

Application

Hot-start PCR amplification

Specific amplification of complex cDNA and genomic template
Amplification from low copy number DNA template

Real-Time PCR
Multiple PCR

Generation of PCR products for TA cloning, Gene microarray analysis and SNP test

Order

Anti body modifed Hotstar DNA Polymerase, suitable for IVD use

Double Anti body modifed Hotstar DNA Polymerase, suitable for IVD use

Contacts

Tinzyme Co., Limited

Email

We will reply as soon as possible

Phone

Mon - Sun 08:00 - 20:00

Copyright 2022 Tinzyme Ltd. All Rights Reserved.